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Multiple Choice
In the process of performing a capsular stain, at what stage should heat fixation be applied?
A
Heat fixation is not used in capsular staining
B
After applying the primary stain
C
After applying the counterstain
D
Before applying the primary stain
Verified step by step guidance
1
Understand the purpose of a capsular stain: It is used to visualize the capsule of certain bacteria, which is a gelatinous outer layer that can be important for pathogenicity.
Recognize that heat fixation is typically used in staining procedures to kill the bacteria and fix them to the slide. However, it can also shrink or destroy the capsule, which is not desirable in capsular staining.
Identify the steps in a typical capsular staining procedure: The primary stain is applied to color the cell and the background, while the capsule remains unstained and appears as a clear halo.
Note that heat fixation is not used in capsular staining because it can damage or destroy the capsule, making it invisible under the microscope.
Conclude that in capsular staining, the procedure is designed to avoid heat fixation to preserve the integrity of the capsule for observation.