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Multiple Choice
Which stain is commonly used to stain the cytoplasm of cells in a capsule stain procedure?
A
Safranin
B
Crystal Violet
C
Methylene Blue
D
Nigrosin
Verified step by step guidance
1
Understand the purpose of a capsule stain procedure: It is used to visualize the capsule of bacteria, which is a protective layer that surrounds some bacterial cells.
Recognize that the capsule itself does not take up most stains, so the procedure involves staining the background and the cytoplasm to create contrast.
Identify the role of different stains: Nigrosin is often used to stain the background, creating a dark field around the cells.
Learn that Methylene Blue is commonly used to stain the cytoplasm of cells, providing a contrast against the unstained capsule.
Note that Safranin and Crystal Violet are not typically used for staining the cytoplasm in capsule stain procedures; they are more common in other types of staining like Gram staining.