Table of contents

1. Introduction to Biochemistry4h 34m
2. Water3h 23m
3. Amino Acids8h 10m
4. Protein Structure10h 4m
5. Protein Techniques14h 5m
6. Enzymes and Enzyme Kinetics13h 38m
7. Enzyme Inhibition and Regulation 8h 42m
8. Protein Function 9h 41m
9. Carbohydrates7h 49m
10. Lipids5h 49m
11. Biological Membranes and Transport 6h 37m
12. Biosignaling9h 45m
Review 1: Nucleic Acids, Lipids, & Membranes2h 47m
Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway3h 12m
Review 3: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism2h 26m
Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation1h 48m

6. Enzymes and Enzyme Kinetics

Enzyme Kinetics

Biochemistry

6. Enzymes and Enzyme Kinetics

Enzyme Kinetics

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Multiple Choice

Which of the following is a method commonly used to measure the activity of an enzyme?

Spectrophotometric assay

Polymerase chain reaction (PCR)

Gel electrophoresis

Western blotting

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Verified step by step guidance

Step 1: Understand the concept of enzyme activity measurement. Enzyme activity refers to the rate at which an enzyme catalyzes a reaction. Measuring enzyme activity often involves monitoring changes in substrate or product concentration over time.

Step 2: Learn about spectrophotometric assays. Spectrophotometric assays are commonly used to measure enzyme activity because they allow for the detection of changes in absorbance or fluorescence of a substrate or product during the reaction. This method is quantitative and efficient.

Step 3: Differentiate spectrophotometric assays from other techniques listed. Polymerase chain reaction (PCR) is used for amplifying DNA, gel electrophoresis is used for separating macromolecules like DNA or proteins, and Western blotting is used for detecting specific proteins. None of these methods directly measure enzyme activity.

Step 4: Recognize why spectrophotometric assays are preferred. They are widely used because they provide real-time data on reaction kinetics, are relatively simple to perform, and can be adapted for various enzymes and substrates.

Step 5: Conclude that spectrophotometric assays are the correct method for measuring enzyme activity in this context, as they are specifically designed for this purpose.