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Multiple Choice
The enzymes in the replisome are active on which of the following?
A
Only the lagging strand
B
Both the leading and lagging strands
C
Only the template strand
D
Only the leading strand
Verified step by step guidance
1
Understand the role of the replisome: The replisome is a complex of enzymes responsible for DNA replication. It includes helicase, primase, DNA polymerase, and other proteins that work together to replicate both strands of DNA simultaneously.
Recall the structure of DNA: DNA is double-stranded, with one strand serving as the leading strand and the other as the lagging strand during replication. Both strands act as templates for synthesis.
Differentiate between leading and lagging strand synthesis: The leading strand is synthesized continuously in the 5' to 3' direction, while the lagging strand is synthesized discontinuously in short fragments called Okazaki fragments, also in the 5' to 3' direction.
Identify the enzymes involved: DNA polymerase synthesizes new DNA on both strands, helicase unwinds the DNA, and primase lays down RNA primers on both strands. These enzymes are active on both the leading and lagging strands during replication.
Conclude that the replisome functions on both strands: Since the replisome contains enzymes that are active on both the leading and lagging strands, the correct answer is 'Both the leading and lagging strands.'