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Multiple Choice
Which enzyme is responsible for covalently attaching methyl groups to DNA?
A
DNA methyltransferase
B
Topoisomerase
C
DNA ligase
D
DNA polymerase
Verified step by step guidance
1
Understand the role of methylation in DNA: Methylation involves the addition of methyl groups (-CH₃) to specific bases in DNA, typically cytosine residues in CpG sites. This process is crucial for regulating gene expression and maintaining epigenetic marks.
Identify the enzyme responsible for methylation: The enzyme that catalyzes the transfer of methyl groups to DNA is known as DNA methyltransferase. It uses S-adenosylmethionine (SAM) as the methyl donor.
Differentiate between the given options: Topoisomerase is involved in relieving supercoiling in DNA during replication or transcription. DNA ligase is responsible for joining DNA strands by forming phosphodiester bonds. DNA polymerase synthesizes new DNA strands during replication. None of these enzymes are involved in methylation.
Focus on DNA methyltransferase: DNA methyltransferase specifically recognizes target sequences in DNA and covalently attaches methyl groups to cytosine bases, altering the DNA's epigenetic state.
Conclude that DNA methyltransferase is the correct answer: Based on its function in methylation, DNA methyltransferase is the enzyme responsible for covalently attaching methyl groups to DNA.