Table of contents

1. Introduction to Biochemistry4h 34m
2. Water3h 23m
3. Amino Acids8h 10m
4. Protein Structure10h 4m
5. Protein Techniques14h 5m
6. Enzymes and Enzyme Kinetics13h 38m
7. Enzyme Inhibition and Regulation 8h 42m
8. Protein Function 9h 41m
9. Carbohydrates7h 49m
10. Lipids5h 49m
11. Biological Membranes and Transport 6h 37m
12. Biosignaling9h 45m
Review 1: Nucleic Acids, Lipids, & Membranes2h 47m
Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway3h 12m
Review 3: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism2h 26m
Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation1h 48m

3. Amino Acids

Isoelectric Point

Biochemistry

3. Amino Acids

Isoelectric Point

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Multiple Choice

Which of the following properties would most likely result in a protein having the largest negative net charge at pH 7?

A low isoelectric point (pI) due to a high content of acidic amino acids

A high content of nonpolar amino acids

A pI close to 7 with equal numbers of acidic and basic residues

A high isoelectric point (pI) due to a high content of basic amino acids

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Verified step by step guidance

Understand the concept of isoelectric point (pI): The isoelectric point is the pH at which a protein has no net charge. Proteins with a low pI are rich in acidic amino acids, while proteins with a high pI are rich in basic amino acids.

Recall the relationship between pH and net charge: At pH 7, proteins with a low pI (below 7) will have a negative net charge because the acidic amino acids will be deprotonated, contributing negatively charged groups.

Analyze the options: Proteins with a high content of acidic amino acids will have a low pI and are more likely to have a negative net charge at pH 7. Proteins with a high content of basic amino acids will have a high pI and a positive net charge at pH 7.

Eliminate irrelevant options: A high content of nonpolar amino acids does not significantly affect the net charge at pH 7, as nonpolar residues are neutral. Similarly, a pI close to 7 with equal numbers of acidic and basic residues will result in a near-neutral charge at pH 7.

Conclude: The property most likely to result in a protein having the largest negative net charge at pH 7 is a low isoelectric point (pI) due to a high content of acidic amino acids.