Multiple Choice
Lactase and carbonic anhydrase are examples of which type of substance?
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6. Enzymes and Enzyme Kinetics
Enzymes
Struggling with Biochemistry?
Join thousands of students who trust us to help them ace their exams!Watch the first videoMultiple Choice
In most biochemical tests involving enzymes, which parameter is most frequently measured to assess enzyme activity?
A
The pH of the buffer solution
B
The rate of product formation
C
The concentration of substrate at equilibrium
D
The molecular weight of the enzyme
Verified step by step guidance1
Understand the concept of enzyme activity: Enzyme activity refers to the ability of an enzyme to catalyze a reaction, which is typically assessed by measuring how efficiently it converts substrates into products.
Identify the key parameter for enzyme activity: The most common way to measure enzyme activity is by monitoring the rate of product formation over time, as this directly reflects the enzyme's catalytic efficiency.
Eliminate irrelevant options: The pH of the buffer solution is important for maintaining enzyme stability but does not directly measure activity. The concentration of substrate at equilibrium is related to reaction completion, not activity. The molecular weight of the enzyme is a structural property, not an activity parameter.
Relate the rate of product formation to enzyme kinetics: The rate of product formation is often measured using spectrophotometry, fluorometry, or other techniques that track changes in product concentration over time.
Conclude that the rate of product formation is the most relevant parameter for assessing enzyme activity, as it provides direct insight into the enzyme's functional performance in catalyzing a reaction.
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