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Which sequence of steps correctly illustrates the process of collecting and purifying proteins using column chromatography?
In cation exchange chromatography, what type of charge does the stationary phase carry, and why is it important?
Which of the following is a limitation of using anion-exchange chromatography for protein purification?
What does the first peak on a chromatogram typically represent in size exclusion chromatography?
Why might a researcher choose to use salt instead of soluble ligands for eluting proteins in affinity chromatography?
What does achieving maximal and constant specific activity signify in protein purification?
What role does high pressure play in HPLC?
Which of the following factors would increase the absorbance of a solution in a spectrophotometer?
What are the benefits and challenges of using native gel electrophoresis for protein analysis?
An unknown protein band is located between markers of 70,000 and 60,000 Da. What is the estimated molecular weight of the protein?
Which component of SDS-PAGE is responsible for denaturing proteins?
What role do ampholytes play in establishing a stable pH gradient in isoelectric focusing?
What is a key advantage of using 2D electrophoresis over isoelectric focusing or SDS PAGE alone?
Which of the following sequences correctly outlines the steps of diagonal electrophoresis?
How does mass spectrometry contribute to understanding complex molecules like proteins?
If a mass spectrum shows peaks at m/z 450 and m/z 550, what is the mass of the amino acid residue between these peaks?
Which component of tandem mass spectrometry allows for the analysis of larger proteins?
What makes peptide mass fingerprinting an efficient method for protein identification?
How does HPLC contribute to protein sequencing?
How does 6 molar hydrochloric acid speed up the hydrolysis of peptide bonds?
How can the analysis of DNP derivatives using HPLC help determine the number of subunits in a protein?
What is the impact of disulfide bonds on protein sequencing?
Which statement accurately compares trypsin and chymotrypsin specificity?
What role does HPLC play in the Edman degradation process?
A peptide sequence is determined using Edman degradation and HPLC. If the chromatogram shows peaks for alanine, glycine, and valine, what is the sequence from the N-terminal to the C-terminal?
Why is it necessary to cleave large proteins into smaller fragments for Edman degradation?