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Multiple Choice
Which of the following methods is useful for studying mRNA expression in different tissues?
A
Chromatin immunoprecipitation
B
Northern blotting
C
Western blotting
D
DNA sequencing
Verified step by step guidance
1
Understand the purpose of each method: Chromatin immunoprecipitation is used to study protein-DNA interactions, Western blotting is used to detect specific proteins, and DNA sequencing is used to determine the nucleotide sequence of DNA. Northern blotting, however, is specifically used to study mRNA expression.
Recognize that Northern blotting involves the separation of RNA samples by gel electrophoresis, followed by transfer to a membrane and detection with a labeled probe that is complementary to the mRNA of interest.
Consider the context of the problem: We are interested in studying mRNA expression, which involves detecting and quantifying specific mRNA molecules in different tissues.
Identify that Northern blotting is the method that allows for the detection and comparison of mRNA levels across different samples, making it suitable for studying mRNA expression in various tissues.
Conclude that Northern blotting is the correct method for studying mRNA expression in different tissues, as it provides information on the presence and quantity of specific mRNA molecules.