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Multiple Choice
Arrange the following steps of DNA replication in the correct order:A. DNA polymerase synthesizes new DNA strandsB. Helicase unwinds the DNA double helixC. Primase synthesizes RNA primersD. Ligase joins Okazaki fragmentsWhich of the following sequences is correct?
A
B → A → C → D
B
C → B → A → D
C
A → B → C → D
D
B → C → A → D
Verified step by step guidance
1
Step 1: Understand the process of DNA replication. DNA replication is a semi-conservative process where the DNA double helix is unwound, and new complementary strands are synthesized using the original strands as templates.
Step 2: Identify the role of each enzyme in DNA replication. Helicase unwinds the DNA double helix, primase synthesizes RNA primers, DNA polymerase synthesizes new DNA strands, and ligase joins Okazaki fragments on the lagging strand.
Step 3: Arrange the steps in the correct order based on their function. First, helicase unwinds the DNA double helix to create a replication fork (B). Next, primase synthesizes short RNA primers to provide a starting point for DNA polymerase (C). Then, DNA polymerase extends the new DNA strands by adding nucleotides complementary to the template strand (A). Finally, ligase seals the gaps between Okazaki fragments on the lagging strand (D).
Step 4: Match the sequence of steps to the provided options. The correct sequence is B → C → A → D.
Step 5: Confirm the reasoning by reviewing the roles of the enzymes and the sequence of events in DNA replication. This ensures the steps are logically and biologically accurate.