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Multiple Choice
What genetic information does cDNA contain in the context of the Central Dogma?
A
Protein sequences translated from mRNA
B
RNA sequences transcribed from DNA
C
Original DNA sequences from the genome
D
Complementary DNA sequences derived from mRNA
Verified step by step guidance
1
Understand the Central Dogma of molecular biology, which describes the flow of genetic information: DNA is transcribed into RNA, and RNA is translated into protein.
Recognize that cDNA, or complementary DNA, is synthesized from an mRNA template using the enzyme reverse transcriptase. This process is called reverse transcription.
Note that cDNA contains only the exons of a gene, as it is derived from mRNA, which has already undergone splicing to remove introns.
Realize that cDNA does not contain the original DNA sequences from the genome, nor does it contain RNA sequences. It is a DNA copy of the mRNA sequence.
Conclude that cDNA is used in various applications, such as cloning eukaryotic genes in prokaryotes, because it represents the coding sequence of a gene without introns.