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Multiple Choice
What type of microscope uses an annular stop to enhance contrast in unstained specimens?
A
Electron microscope
B
Fluorescence microscope
C
Phase-contrast microscope
D
Confocal microscope
Verified step by step guidance
1
Understand the purpose of the microscope: The question asks about a microscope that enhances contrast in unstained specimens. This is important because some specimens cannot be stained or are best observed in their natural state.
Identify the key feature mentioned: The term 'annular stop' is crucial here. An annular stop is a component used in certain microscopes to manipulate light paths and enhance contrast.
Recall the function of different microscopes: Electron microscopes use electron beams and are not typically used for unstained specimens. Fluorescence microscopes rely on fluorescent dyes, which require staining. Confocal microscopes use laser light to scan specimens, often requiring staining for better visualization.
Focus on phase-contrast microscopy: Phase-contrast microscopes are specifically designed to enhance contrast in unstained specimens. They use an annular stop to create phase shifts in light passing through the specimen, making transparent structures more visible.
Conclude with the correct choice: Based on the function of the annular stop and the need to enhance contrast in unstained specimens, the phase-contrast microscope is the correct answer.