Multiple Choice
Which of the following can be used to cleave DNA?
3
views
18. Biotechnology
Introduction to DNA-Based Technology
Struggling with General Biology?
Join thousands of students who trust us to help them ace their exams!Watch the first videoMultiple Choice
Which aspect of the polymerase chain reaction (PCR) is most frequently falsified in scientific misconduct cases?
A
The choice of DNA polymerase enzyme
B
The temperature of the denaturation step
C
The design of primer sequences
D
The reporting of gel electrophoresis results
Verified step by step guidance1
Understand the context of the problem: The polymerase chain reaction (PCR) is a widely used technique in molecular biology to amplify DNA sequences. Scientific misconduct in PCR often involves falsifying data related to the results of the process.
Identify the key components of PCR: PCR involves three main steps—denaturation, annealing, and extension. It also requires specific reagents such as DNA polymerase, primers, and a template DNA sequence.
Focus on the aspect mentioned in the problem: The reporting of gel electrophoresis results is highlighted as the most frequently falsified aspect. Gel electrophoresis is used to visualize the amplified DNA fragments after PCR, providing evidence of successful amplification.
Explain why gel electrophoresis results are prone to falsification: Researchers may manipulate or fabricate gel images to falsely claim successful amplification of DNA, which can mislead others about the validity of their experiments.
Conclude with the importance of ethical practices: Emphasize that accurate reporting of gel electrophoresis results is crucial for scientific integrity, as it ensures reproducibility and reliability of PCR-based experiments.
2:30mWatch next
Master Introduction to DNA-Based Technology with a bite sized video explanation from Jason
Start learningRelated Videos
Related Practice
Introduction to DNA-Based Technology practice set
Problem sets built by lead tutorsExpert video explanations