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Multiple Choice
Which two types of microscopes are commonly used to generate three-dimensional images of specimens?
A
Phase-contrast microscope and darkfield microscope
B
Scanning electron microscope (SEM) and confocal laser scanning microscope
C
Transmission electron microscope (TEM) and brightfield light microscope
D
Fluorescence microscope and compound light microscope
Verified step by step guidance
1
Understand the purpose of the question: The goal is to identify which types of microscopes are commonly used to generate three-dimensional images of specimens.
Recall the characteristics of each microscope type: Scanning electron microscope (SEM) uses electron beams to scan the surface of specimens, producing detailed 3D images. Confocal laser scanning microscope uses lasers and optical sectioning to create 3D reconstructions of specimens.
Eliminate incorrect options: Phase-contrast and darkfield microscopes are used for enhancing contrast in unstained specimens but do not produce 3D images. Transmission electron microscope (TEM) provides high-resolution 2D images, not 3D. Brightfield light microscope is used for basic imaging but lacks 3D capabilities. Fluorescence and compound light microscopes are used for specific imaging techniques but do not generate 3D images.
Focus on the correct answer: Scanning electron microscope (SEM) and confocal laser scanning microscope are specifically designed to generate three-dimensional images of specimens.
Conclude with the reasoning: SEM provides surface-level 3D imaging, while confocal laser scanning microscope allows for optical sectioning and 3D reconstruction, making them the correct answer for this question.