Multiple Choice
How are monoclonal antibodies produced in DNA-based technology?
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18. Biotechnology
Introduction to DNA-Based Technology
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Join thousands of students who trust us to help them ace their exams!Watch the first videoMultiple Choice
Which of the following is a step in the process of producing transgenic bacteria?
A
Isolation of bacterial ribosomes
B
Replication of bacterial DNA without modification
C
Transcription of bacterial RNA
D
Insertion of foreign DNA into a bacterial plasmid
Verified step by step guidance1
Understand the concept of transgenic bacteria: Transgenic bacteria are those that have been genetically modified to contain foreign DNA, which allows them to express new traits or produce new substances.
Identify the role of plasmids: Plasmids are small, circular DNA molecules found in bacteria that can replicate independently of chromosomal DNA. They are often used as vectors to introduce foreign DNA into bacteria.
Recognize the process of inserting foreign DNA: The insertion of foreign DNA into a bacterial plasmid involves cutting both the plasmid and the foreign DNA with the same restriction enzyme to create compatible ends.
Learn about the ligation process: The foreign DNA is then ligated, or joined, to the plasmid DNA using an enzyme called DNA ligase, forming a recombinant plasmid.
Understand the transformation step: The recombinant plasmid is introduced into bacterial cells through a process called transformation, where the bacteria take up the plasmid and express the foreign gene.
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