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Multiple Choice
What is the function of the enzyme ligase during DNA replication?
A
It synthesizes RNA primers.
B
It adds nucleotides to the growing DNA strand.
C
It unwinds the DNA double helix.
D
It joins Okazaki fragments on the lagging strand.
Verified step by step guidance
1
Understand the context of DNA replication: DNA replication is the process by which a cell duplicates its DNA, resulting in two identical DNA molecules. This process involves several enzymes, each with a specific function.
Identify the role of DNA ligase: DNA ligase is an enzyme that plays a crucial role in DNA replication, particularly on the lagging strand.
Recognize the structure of the lagging strand: During DNA replication, the lagging strand is synthesized in short segments known as Okazaki fragments. These fragments are created because DNA polymerase can only synthesize DNA in the 5' to 3' direction.
Explain the function of DNA ligase: DNA ligase is responsible for joining the Okazaki fragments together. It does this by catalyzing the formation of phosphodiester bonds between the 3' hydroxyl end of one fragment and the 5' phosphate end of the next fragment.
Summarize the importance of DNA ligase: By joining the Okazaki fragments, DNA ligase ensures that the lagging strand becomes a continuous DNA strand, which is essential for the integrity and stability of the newly synthesized DNA molecule.