Which of the following must occur for speciation to happen?

The red wolf, Canis rufus, which was once widespread in the southeastern and south central United States, was declared extinct in the wild by 1980. Saved by a captive breeding program, the red wolf has been reintroduced in areas of eastern North Carolina. The current wild population is estimated to be about 100 individuals. It is presently being threatened with extinction due to hybridization with coyotes, Canis latrans, which have become more numerous in the area. Red wolves and coyotes differ in terms of morphology, DNA, and behavior, although these differences may disappear if interbreeding continues. Although the red wolf has been designated as an endangered species under the Endangered Species Act, some people think that its endangered status should be withdrawn and resources should not be spent to protect what is not a 'pure' species. Do you agree? Why or why not?

Which of the following is an example of temporal isolation in speciation?

Why is it that two species cannot occupy the same niche for an extended period of time?

Which of the following is an example of reproductive isolation?

Which principle states that two species in the same habitat cannot occupy the same niche?

Which of these is an example of temporal isolation between two species?