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Multiple Choice
Why must atmospheric nitrogen ($N_2$) be 'fixed' before most organisms can use it, and which organisms are primarily responsible for this process?
A
Because $N_2$ is highly reactive and must be stabilized by fungi before use.
B
Because $N_2$ is inert and cannot be directly used by most living organisms; nitrogen-fixing bacteria convert it into usable forms.
C
Because $N_2$ is toxic to most organisms; only plants can convert it into ammonia.
D
Because $N_2$ is only present in water; algae are responsible for nitrogen fixation.
Verified step by step guidance
1
Understand the nature of atmospheric nitrogen ($N_2$): Atmospheric nitrogen is a diatomic molecule with a triple bond between the two nitrogen atoms. This bond is extremely strong, making $N_2$ chemically inert and not readily usable by most organisms.
Recognize why nitrogen fixation is necessary: Most organisms cannot break the triple bond in $N_2$ to access nitrogen for building essential biomolecules like amino acids and nucleotides. Nitrogen must be converted into more reactive and usable forms, such as ammonia ($NH_3$) or nitrate ($NO_3^-$).
Identify the organisms responsible for nitrogen fixation: Nitrogen-fixing bacteria, such as those in the genera *Rhizobium* and *Azotobacter*, are primarily responsible for converting $N_2$ into usable forms. Some cyanobacteria also perform nitrogen fixation.
Understand the process of nitrogen fixation: These bacteria use the enzyme nitrogenase to catalyze the conversion of $N_2$ into ammonia ($NH_3$). The reaction requires energy, typically supplied by ATP, and occurs under anaerobic conditions to protect the nitrogenase enzyme from oxygen damage.
Recognize the ecological importance: Nitrogen fixation is a critical process in the nitrogen cycle, enabling the incorporation of atmospheric nitrogen into ecosystems. Plants often form symbiotic relationships with nitrogen-fixing bacteria to access fixed nitrogen directly.