Understand the process of carbon fixation in C3 plants: In C3 plants, the Calvin cycle is the primary pathway for carbon fixation. The enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) catalyzes the reaction between CO$_2$ and ribulose-1,5-bisphosphate (RuBP), a 5-carbon molecule.