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Multiple Choice
What function does the enzyme DNA ligase perform in DNA replication?
A
It unwinds the DNA double helix.
B
It synthesizes new DNA strands by adding nucleotides.
C
It joins Okazaki fragments on the lagging strand.
D
It removes RNA primers and replaces them with DNA.
Verified step by step guidance
1
Understand the role of DNA ligase in DNA replication: DNA ligase is an enzyme that plays a crucial role in the process of DNA replication, particularly on the lagging strand.
Recognize the structure of the lagging strand: During DNA replication, the lagging strand is synthesized in short segments known as Okazaki fragments.
Identify the problem with Okazaki fragments: These fragments are initially separate and need to be joined together to form a continuous DNA strand.
Learn the specific function of DNA ligase: DNA ligase catalyzes the formation of phosphodiester bonds between the 3' hydroxyl end of one nucleotide and the 5' phosphate end of another, effectively joining the Okazaki fragments.
Conclude the role of DNA ligase: By joining the Okazaki fragments, DNA ligase ensures the integrity and continuity of the newly synthesized DNA strand on the lagging side.