Textbook Question
The ultrastructure of a chloroplast is best studied using a
a. Light microscope
b. Scanning electron microscope
c. Transmission electron microscope
d. Light microscope and fluorescent dyes
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5. Cell Components
Microscopes
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Why is it easier to locate your specimen using a lower power objective on a microscope?
A
Lower power objectives require less light.
B
Lower power objectives have higher magnification.
C
Lower power objectives provide a wider field of view.
D
Lower power objectives have a smaller depth of field.
Verified step by step guidance1
Understand the concept of 'field of view' in microscopy: The field of view is the visible area seen through the microscope eyepiece. It is the extent of the observable world that is seen at any given moment.
Recognize the relationship between magnification and field of view: As magnification increases, the field of view decreases. This means that higher magnification lenses show a smaller area of the specimen.
Identify the characteristics of lower power objectives: Lower power objectives have lower magnification, which results in a larger field of view. This allows you to see more of the specimen at once.
Consider the practical implications: With a wider field of view, it is easier to locate and center the specimen on the slide because you can see more of the surrounding area.
Conclude why lower power objectives are advantageous for initial specimen location: The wider field of view provided by lower power objectives makes it easier to find and focus on the specimen, especially when initially setting up the microscope.
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