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Multiple Choice
During the light reactions of photosynthesis, at what specific stage is oxygen ($O_2$) produced?
A
When carbon dioxide ($CO_2$) is fixed in the Calvin cycle
B
When electrons are transferred from photosystem I to NADP$^+$
C
During the synthesis of ATP by ATP synthase
D
When water ($H_2O$) is split by photosystem II
Verified step by step guidance
1
Understand the context of the light reactions in photosynthesis: These reactions occur in the thylakoid membranes of chloroplasts and involve the conversion of light energy into chemical energy, producing ATP and NADPH while releasing oxygen ($O_2$).
Identify the role of photosystem II: Photosystem II is the first protein complex in the light reactions. It absorbs light energy, which excites electrons and initiates the electron transport chain.
Recognize the process of water splitting (photolysis): Photosystem II uses light energy to split water molecules ($H_2O$) into protons ($H^+$), electrons, and oxygen ($O_2$). This process replenishes the electrons lost by photosystem II during excitation.
Understand the production of oxygen ($O_2$): The splitting of water by photosystem II releases oxygen as a byproduct. This is the specific stage where oxygen is produced during the light reactions.
Clarify why other options are incorrect: Oxygen is not produced during carbon fixation in the Calvin cycle, electron transfer from photosystem I to NADP$^+$, or ATP synthesis by ATP synthase. These processes are distinct from the water-splitting step in photosystem II.