Multiple Choice
At which of the following levels can gene expression be regulated in eukaryotes?
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16. Regulation of Expression
Introduction to Regulation of Gene Expression
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Which technique cannot be used to analyze gene expression?
A
RT-PCR
B
RNA sequencing
C
Microarray analysis
D
Western blotting
Verified step by step guidance1
Understand the concept of gene expression analysis, which involves measuring the activity of genes to understand their function and regulation.
RT-PCR (Reverse Transcription Polymerase Chain Reaction) is a technique used to amplify and quantify RNA, making it suitable for analyzing gene expression.
RNA sequencing is a method that allows for the comprehensive analysis of the entire transcriptome, providing insights into gene expression levels across different conditions.
Microarray analysis involves using a grid of DNA probes to measure the expression levels of thousands of genes simultaneously, making it a powerful tool for gene expression profiling.
Western blotting is a technique used to detect specific proteins in a sample, not RNA or gene expression. It involves separating proteins by gel electrophoresis, transferring them to a membrane, and using antibodies for detection, which is why it cannot be used for analyzing gene expression.
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