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Multiple Choice
PCR and cellular DNA replication share which of the following characteristics?
A
Both involve the enzyme helicase to unwind the DNA double helix.
B
Both utilize RNA primers to initiate DNA synthesis.
C
Both require a DNA template to synthesize new DNA strands.
D
Both occur in the nucleus of eukaryotic cells.
Verified step by step guidance
1
Understand the process of PCR (Polymerase Chain Reaction): PCR is a laboratory technique used to amplify DNA sequences. It involves repeated cycles of denaturation, annealing, and extension to create multiple copies of a specific DNA segment.
Understand cellular DNA replication: This is a natural process occurring in living cells where the DNA double helix is unwound and each strand serves as a template for the synthesis of a new complementary strand.
Identify the role of a DNA template: In both PCR and cellular DNA replication, a DNA template is required. This template provides the sequence that will be copied to form new DNA strands.
Compare the use of enzymes: In cellular DNA replication, helicase is used to unwind the DNA double helix. However, in PCR, heat is used to denature the DNA, so helicase is not involved.
Compare the initiation of DNA synthesis: Both processes require primers to initiate DNA synthesis. In cellular replication, RNA primers are used, while in PCR, synthetic DNA primers are used.