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Multiple Choice
Which of the following is true of the analysis of DNA fragments using gel electrophoresis?
A
DNA fragments are separated based on their nucleotide sequence.
B
Gel electrophoresis can only be used to analyze RNA fragments.
C
Smaller DNA fragments move faster and travel further through the gel.
D
Larger DNA fragments move faster and travel further through the gel.
Verified step by step guidance
1
Understand the principle of gel electrophoresis: It is a technique used to separate DNA fragments based on size. DNA fragments are negatively charged due to their phosphate backbone, and when an electric current is applied, they move towards the positive electrode.
Recognize that the gel used in electrophoresis is typically made of agarose or polyacrylamide, which acts as a molecular sieve. Smaller fragments can navigate through the pores of the gel more easily than larger ones.
Consider the statement 'Smaller DNA fragments move faster and travel further through the gel.' This is true because smaller fragments encounter less resistance and can move more quickly through the gel matrix.
Evaluate the statement 'Larger DNA fragments move faster and travel further through the gel.' This is false because larger fragments experience more resistance and move slower, thus traveling a shorter distance.
Conclude that gel electrophoresis separates DNA fragments based on size, not nucleotide sequence, and it can be used for both DNA and RNA analysis, although the problem specifically refers to DNA.