One of the most important properties of proper scientific investigations is their repeatability. Yet, as discussed in Module 32.11, studies that compare the nutritional content of conventional and organic produce sometimes produce contradictory results. Name some possible confounding factors that can account for such uneven results.

There is a conflict between van Helmont's data on willow tree growth and the data on essential nutrients listed in Table 36.1. According to the table, nutrients other than C, H, and O should make up about 4 percent of a willow tree's mass. Most or all of these nutrients should come from soil. But van Helmont claimed that the soil in his experiment lost just 60 g, while the tree gained 74,000 g. Calculate the percentage of the added mass accounted for by soil, and compare it to the predicted 4 percent. State at least one hypothesis to explain the conflict between expected and observed results. How would you test this hypothesis?

Draw a simple sketch of cation exchange, showing a root hair, a soil particle with anions, and a hydrogen ion displacing a mineral cation.

Acid rain contains an excess of hydrogen ions (H⁺). One effect of acid rain is to deplete the soil of plant nutrients such as calcium (Ca²⁺), potassium (K⁺), and magnesium (Mg²⁺).

Offer a hypothesis to explain why acid rain washes these nutrients from the soil.

How might you test your hypothesis?

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Eating even a single death cap mushroom (Amanita phalloides) can be fatal due to a compound called α-amanitin, a toxin that inhibits transcription.

Toxins like α-amanitin are used for research in much the same way as null mutants (Chapter 16)—to disrupt a process and see what happens when it no longer works. Researchers examined the ability of α-amanitin to inhibit different RNA polymerases. They purified RNA polymerases I, II, and III from rat liver, incubated the enzymes with different concentrations of α-amanitin, and then tested their activity. The results of this experiment are shown here. These findings suggest that cells treated with α-amanitin will have a reduced level of:

a. tRNAs

b. rRNAs

c. snRNAs

d. mRNAs

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Eating even a single death cap mushroom (Amanita phalloides) can be fatal due to a compound called α-amanitin, a toxin that inhibits transcription.

If you wanted to use α-amanitin to shut down 95 percent of transcription by RNA polymerase II, roughly what concentration of α-amanitin would you use? Note that the scale on the x-axis of the graph in Question 13 is logarithmic rather than linear, so that each tick mark shows a tenfold higher concentration.