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Multiple Choice
In eukaryotic cells, does glycolysis take place in the mitochondria?
A
Yes; glycolysis occurs in the mitochondrial matrix.
B
No; glycolysis occurs in the cytosol.
C
Yes; glycolysis occurs in the mitochondrial intermembrane space.
D
No; glycolysis occurs in the nucleus.
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1
Recall the cellular compartments involved in metabolic pathways in eukaryotic cells, focusing on where glycolysis occurs.
Understand that glycolysis is the process of breaking down glucose into pyruvate, producing ATP and NADH, and it happens in the cytosol, which is the fluid portion of the cytoplasm.
Recognize that mitochondria are the site of subsequent steps in cellular respiration, such as the Krebs cycle (in the mitochondrial matrix) and oxidative phosphorylation (across the inner mitochondrial membrane), but not glycolysis.
Eliminate options that place glycolysis in the mitochondria (matrix or intermembrane space) or nucleus, since these are incorrect locations for glycolysis.
Conclude that the correct location for glycolysis in eukaryotic cells is the cytosol, outside the mitochondria.