Multiple Choice
Which cell types are derived from primary tissue?
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22. Techniques in Cell Biology
Cell Culture
Multiple Choice
In mammalian cell culture, what does a culture medium primarily provide to living cells to support their survival and growth?
A
Essential nutrients, appropriate ions/osmolarity, buffering to maintain pH, and (often) growth factors
B
Direct ATP production by supplying mitochondria with ready-made ATP to bypass metabolism
C
Genetic instructions (DNA) needed for transcription and translation of all cellular proteins
D
A solid extracellular matrix that permanently anchors cells and replaces the need for cell adhesion molecules
Verified step by step guidance1
Understand that mammalian cells in culture require a supportive environment that mimics their natural conditions to survive and grow.
Recognize that the culture medium primarily provides essential nutrients such as amino acids, vitamins, glucose, and salts, which are necessary for cellular metabolism and biosynthesis.
Note that the medium maintains appropriate ion concentrations and osmolarity to ensure cellular homeostasis and prevent osmotic stress.
Consider that buffering agents in the medium help maintain a stable pH, which is critical for enzyme function and overall cell health.
Acknowledge that many culture media also include growth factors or serum components that stimulate cell division and differentiation, supporting cell proliferation.
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