Multiple Choice
In prokaryotic cells, where is the cell’s DNA primarily located?
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7. DNA and Chromosome Structure
Bacterial and Viral Chromosome Structure
Multiple Choice
In most prokaryotes, how is chromosomal DNA primarily packaged within the cell?
A
As RNA–DNA hybrids packaged inside an icosahedral capsid until cell division
B
As a nucleoid: a highly supercoiled circular chromosome organized by nucleoid-associated proteins (e.g., HU, IHF) without a membrane-bound nucleus
C
As multiple chromosomes condensed by protamines into a tightly packed sperm-like chromatin structure
D
As linear chromosomes wrapped around histone octamers to form nucleosomes within a membrane-bound nucleus
Verified step by step guidance1
Understand that prokaryotic cells lack a membrane-bound nucleus, so their chromosomal DNA is not enclosed within a nuclear membrane.
Recognize that prokaryotic DNA is typically organized into a single, circular chromosome rather than multiple linear chromosomes.
Learn that this circular chromosome is highly supercoiled to efficiently compact the DNA within the limited space of the cell.
Identify the role of nucleoid-associated proteins (such as HU and IHF) which help organize and stabilize the supercoiled DNA structure, forming the nucleoid region.
Conclude that the chromosomal DNA in prokaryotes is packaged as a nucleoid: a highly supercoiled circular chromosome organized by nucleoid-associated proteins, without a membrane-bound nucleus.
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