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Multiple Choice
In the context of transcription, what does the enzyme RNA polymerase do?
A
Splices introns out of pre-mRNA to produce mature mRNA
B
Synthesizes an RNA strand by adding ribonucleotides complementary to the DNA template strand (catalyzes phosphodiester bond formation)
C
Joins Okazaki fragments together during DNA replication
D
Translates mRNA codons into an amino acid sequence to form a polypeptide
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1
Understand the role of RNA polymerase in transcription, which is the process of synthesizing RNA from a DNA template.
Recall that transcription involves creating an RNA strand complementary to the DNA template strand, using ribonucleotides.
Recognize that RNA polymerase catalyzes the formation of phosphodiester bonds between ribonucleotides, linking them together to form the RNA strand.
Differentiate RNA polymerase's function from other processes such as splicing (removal of introns), DNA replication (joining Okazaki fragments), and translation (protein synthesis).
Conclude that RNA polymerase's primary role is to synthesize RNA by adding ribonucleotides complementary to the DNA template strand during transcription.