Multiple Choice
During DNA replication, which protein stabilizes the unwound DNA strands by binding to single-stranded DNA and preventing reannealing?
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8. DNA Replication
Overview of DNA Replication
Multiple Choice
In the overview of DNA replication, which enzyme is primarily responsible for unzipping (unwinding) the DNA double helix at the replication fork to prepare for replication?
A
DNA polymerase III
B
Primase
C
DNA helicase
D
DNA ligase
Verified step by step guidance1
Understand the role of each enzyme listed in the context of DNA replication.
Recall that DNA polymerase III is mainly responsible for adding nucleotides to the growing DNA strand during replication, not for unwinding the helix.
Recognize that primase synthesizes RNA primers to initiate DNA synthesis but does not unwind the DNA.
Identify that DNA ligase joins Okazaki fragments on the lagging strand but does not separate the strands.
Conclude that DNA helicase is the enzyme that unwinds or 'unzips' the DNA double helix at the replication fork, separating the two strands to allow replication to proceed.
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