Multiple Choice
In prokaryotic DNA replication, what is the primary function of DNA polymerase III?
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8. DNA Replication
Overview of DNA Replication
Multiple Choice
In the context of DNA replication, what is the primary function of DNA polymerase?
A
It synthesizes a new DNA strand by adding nucleotides to the 3′ end using the template strand for base-pairing.
B
It unwinds the DNA double helix by breaking hydrogen bonds between base pairs.
C
It seals nicks between Okazaki fragments by forming phosphodiester bonds.
D
It lays down short RNA primers needed to start DNA synthesis.
Verified step by step guidance1
Understand the role of DNA polymerase in DNA replication, which is an enzyme responsible for synthesizing new DNA strands.
Recall that DNA polymerase adds nucleotides to the growing DNA strand by matching them to the complementary bases on the template strand, ensuring accurate base pairing.
Note that DNA polymerase can only add nucleotides to the 3′ end of the new strand, meaning DNA synthesis occurs in the 5′ to 3′ direction.
Differentiate DNA polymerase's function from other enzymes involved in replication, such as helicase (which unwinds the DNA), ligase (which seals nicks), and primase (which lays down RNA primers).
Conclude that the primary function of DNA polymerase is to synthesize a new DNA strand by adding nucleotides to the 3′ end using the template strand for base-pairing.
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