Multiple Choice
Which of the following structures can be involved in genetic recombination?
8. DNA Replication
Recombination
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Which one of the following bacterial matings is most likely to yield a recombinant cell?
A
An F- cell mating with another F- cell
B
An Hfr cell mating with another Hfr cell
C
An Hfr cell mating with an F- cell
D
An F+ cell mating with another F+ cell
Verified step by step guidance1
Understand the types of bacterial cells involved in conjugation: F- cells lack the fertility factor (F plasmid), F+ cells carry the F plasmid as an independent plasmid, and Hfr cells have the F factor integrated into their chromosome.
Recall that bacterial conjugation involves transfer of genetic material from a donor cell to a recipient cell through direct contact, typically from an F+ or Hfr donor to an F- recipient.
Analyze each mating pair: F- × F- involves two recipients with no donor, so no conjugation or recombination occurs; Hfr × Hfr involves two donors, which generally do not transfer DNA to each other; F+ × F+ involves two donors, also unlikely to result in recombination.
Recognize that an Hfr cell mating with an F- cell allows the Hfr donor to transfer part of its chromosomal DNA into the F- recipient, potentially creating recombinant cells with new genetic combinations.
Conclude that the mating between an Hfr cell and an F- cell is most likely to yield recombinant cells because it involves transfer of chromosomal genes, unlike the other combinations.
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