Multiple Choice
In DNA isolation protocols, detergent is included primarily because it:
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18. Molecular Genetic Tools
Methods for Analyzing DNA
Multiple Choice
In agarose gel electrophoresis, DNA molecules will migrate toward which electrode?
A
Whichever electrode is closest to the sample well, regardless of charge
B
The positive electrode (anode)
C
DNA does not migrate in an electric field because it is electrically neutral
D
The negative electrode (cathode)
Verified step by step guidance1
Understand the basic principle of agarose gel electrophoresis: DNA molecules are placed in wells within an agarose gel and an electric field is applied across the gel.
Recall that DNA molecules have a negatively charged phosphate backbone, which means they will move in response to an electric field toward the positive electrode.
Identify the electrodes: the positive electrode is called the anode, and the negative electrode is called the cathode.
Since DNA is negatively charged, it will migrate toward the positive electrode (anode) during electrophoresis.
Therefore, the correct answer is that DNA molecules migrate toward the positive electrode (anode) in agarose gel electrophoresis.
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