Multiple Choice
In the overview of DNA replication, what is the primary role of DNA ligase?
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8. DNA Replication
Overview of DNA Replication
Multiple Choice
In the overview of DNA replication, what is the primary role of DNA polymerase?
A
It unwinds the double helix by breaking hydrogen bonds between base pairs.
B
It lays down short RNA primers to initiate DNA synthesis.
C
It synthesizes a new DNA strand by adding nucleotides to the 3′ end, extending in the 5′→3′ direction using the template strand.
D
It seals nicks between Okazaki fragments by forming phosphodiester bonds.
Verified step by step guidance1
Understand the overall process of DNA replication, which involves unwinding the DNA, priming the strand, synthesizing new DNA, and joining fragments.
Identify the specific function of DNA polymerase among these steps: it is responsible for synthesizing new DNA strands by adding nucleotides complementary to the template strand.
Recall that DNA polymerase adds nucleotides only to the 3′ end of the growing strand, meaning synthesis occurs in the 5′→3′ direction.
Differentiate DNA polymerase's role from other enzymes: helicase unwinds the DNA, primase lays down RNA primers, and DNA ligase seals nicks between Okazaki fragments.
Conclude that the primary role of DNA polymerase is to extend the new DNA strand by adding nucleotides complementary to the template strand, ensuring accurate DNA replication.
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