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Multiple Choice
In the context of RNA and gene expression studies, what is complementary DNA (cDNA)?
A
DNA synthesized from an RNA template by reverse transcriptase, representing the expressed sequences (often mRNA) of a cell
B
The complete set of noncoding DNA sequences removed from pre-mRNA during splicing (introns)
C
A short RNA molecule that base-pairs with mRNA to block translation (antisense RNA)
D
A double-stranded RNA copy of genomic DNA produced during DNA replication
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Verified step by step guidance
1
Understand that complementary DNA (cDNA) is related to the process of gene expression, where RNA molecules are produced from DNA templates.
Recall that cDNA is synthesized from an RNA template, typically messenger RNA (mRNA), which represents the expressed genes in a cell.
Recognize that the enzyme responsible for synthesizing cDNA from RNA is called reverse transcriptase, which creates a DNA strand complementary to the RNA sequence.
Note that cDNA corresponds only to the expressed sequences because it is made from processed mRNA, which has had noncoding regions (introns) removed during RNA splicing.
Distinguish cDNA from other nucleic acid types by remembering that it is not genomic DNA, antisense RNA, or double-stranded RNA, but rather a DNA copy synthesized from RNA.