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Multiple Choice
Given the DNA template strand 3'-TACGGTACG-5', which strand of mRNA would be synthesized during transcription?
A
5'-ATGCCATGC-3'
B
5'-TACGGTACG-3'
C
3'-AUGCCAUGC-5'
D
5'-AUGCCAUGC-3'
Verified step by step guidance
1
Identify the direction of the DNA template strand given, which is 3'-TACGGTACG-5'. Transcription proceeds by reading the template strand from 3' to 5' to synthesize mRNA in the 5' to 3' direction.
Recall that during transcription, RNA polymerase synthesizes an mRNA strand complementary to the DNA template strand, but with uracil (U) replacing thymine (T).
Write the complementary RNA bases for each DNA base on the template strand: A pairs with U, T pairs with A, C pairs with G, and G pairs with C.
Construct the mRNA strand by pairing each base of the DNA template strand with its RNA complement, ensuring the mRNA strand is oriented 5' to 3'.
Verify the mRNA sequence matches the complementary bases and correct orientation, confirming the synthesized mRNA strand is 5'-AUGCCAUGC-3'.