In land plants, there is an alternation of generations between a haploid gametophyte generation and a diploid sporophytic generation. Both generations are typically multicellular and may be free-living. The male (pollen) and female (embryo sac) gametophytes are the haploid generation of flowering plants.
How would you conduct a screen to identify genes required for female gametophyte development in Arabidopsis?

Homeotic genes are thought to regulate each other. What aspect of the phenotype of apetala2 agamous double mutants indicates that these two genes act antagonistically?

Basidiomycota is a monophyletic group of fungi that includes most of the common mushrooms. You are interested in the development of the body plan of mushrooms. How would you identify the genes required for patterning during mushroom development?

In Drosophila, loss-of-function Ultrabithorax mutations result in the posterior thoracic segments differentiating into body parts with an identity normally found in the anterior thoracic segments. When the Ultrabithorax gene was cloned, it was shown to encode a transcription factor and to be expressed only in the posterior region of the thorax. Thus, Ultrabithorax acts to specify the identity of the posterior thoracic segments. Similar genes were soon discovered in other animals, including mice and humans. You have found that mice possess two closely related genes, Hoxa7 and Hoxb4, which are orthologs of Ultrabithorax. You wish to know whether the two mouse genes act to specify the identity of body segments in mice.

How will you determine whether the mouse genes have redundant functions?

In land plants, there is an alternation of generations between a haploid gametophyte generation and a diploid sporophytic generation. Both generations are typically multicellular and may be free-living. The male (pollen) and female (embryo sac) gametophytes are the haploid generation of flowering plants.

How would you conduct a screen to identify genes required for male gametophyte development?

Zea mays (maize, or corn) was originally domesticated in central Mexico at least 7000 years ago from an endemic grass called teosinte. Teosinte is generally unbranched, has male and female flowers on the same branch, and has few kernels per 'cob,' each encased in a hard, leaf-like organ called a glume. In contrast, maize is highly branched, with a male inflorescence (tassel) on its central branch and female inflorescences (cobs) on axillary branches. In addition, maize cobs have many rows of kernels and soft glumes. George Beadle crossed cultivated maize and wild teosinte, which resulted in fully fertile F₁ plants. When the F₁ plants were self-fertilized, about 1 plant in every 1000 of the F₂ progeny resembled either a modern maize plant or a wild teosinte plant. What did Beadle conclude about whether the different architectures of maize and teosinte were caused by changes with a small effect in many genes or changes with a large effect in just a few genes?

In Drosophila, recessive mutations in the fruitless gene (fru) result in males courting other males, and recessive mutations in the Antennapedia gene (Ant⁻) lead to defects in the body plan, specifically in the thoracic region of the body, where mutants fail to develop legs. The two genes map 15 cM apart on chromosome 3. You have isolated a new dominant Antdᵈ mutant allele that you induced by treating your flies with X-rays. Your new mutant has legs developing instead of antennae on the head of the fly. You cross your newly induced dominant Antᵈ mutant (a pure-breeding line) with a homozygous recessive fru mutant (which is homozygous wild type at the Ant⁺ locus), as diagrammed below:

Provide a molecular explanation for the reason your new Antᵈ mutant is dominant and for its novel phenotype.

In Drosophila, recessive mutations in the fruitless gene (fru) result in males courting other males, and recessive mutations in the Antennapedia gene (Ant⁻) lead to defects in the body plan, specifically in the thoracic region of the body, where mutants fail to develop legs. The two genes map 15 cM apart on chromosome 3. You have isolated a new dominant Antdᵈ mutant allele that you induced by treating your flies with X-rays. Your new mutant has legs developing instead of antennae on the head of the fly. You cross your newly induced dominant Antᵈ mutant (a pure-breeding line) with a homozygous recessive fru mutant (which is homozygous wild type at the Ant⁺ locus), as diagrammed below: Your cross results in the following phenotypic proportions:

Legs on head, normal courting behavior 75

Normal head, abnormal courting behavior 25

Legs on head, abnormal courting behavior 0

Normal head, normal courting behavior 0

Provide a genetic explanation for these results and describe a test for your hypothesis

In Drosophila, recessive mutations in the fruitless gene (fru) result in males courting other males, and recessive mutations in the Antennapedia gene (Ant⁻) lead to defects in the body plan, specifically in the thoracic region of the body, where mutants fail to develop legs. The two genes map 15 cM apart on chromosome 3. You have isolated a new dominant Antdᵈ mutant allele that you induced by treating your flies with X-rays. Your new mutant has legs developing instead of antennae on the head of the fly. You cross your newly induced dominant Antᵈ mutant (a pure-breeding line) with a homozygous recessive fru mutant (which is homozygous wild type at the Ant⁺ locus), as diagrammed below:

What phenotypes, and in what proportions, do you expect in the F₂ obtained by interbreeding F₁ animals?