Har Gobind Khorana and his colleagues performed numerous experiments translating synthetic mRNAs. In one experiment, an mRNA molecule with a repeating UG dinucleotide sequence was assembled and translated.
What is the sequence of the resulting polypeptide?

The amino acid sequence of a portion of a polypeptide is

N...Cys-Pro-Ala-Met-Gly-His-Lys...C

What is the mRNA sequence encoding this polypeptide fragment? Use N to represent any nucleotide, Pu to represent a purine, and Py to represent a pyrimidine. Label the 5′ and 3′ ends of the mRNA.

Har Gobind Khorana and his colleagues performed numerous experiments translating synthetic mRNAs. In one experiment, an mRNA molecule with a repeating UG dinucleotide sequence was assembled and translated.

If the genetic code were overlapping rather than nonoverlapping, how would the result of this experiment be different?

Har Gobind Khorana and his colleagues performed numerous experiments translating synthetic mRNAs. In one experiment, an mRNA molecule with a repeating UG dinucleotide sequence was assembled and translated.

If the genetic code were a doublet code instead of a triplet code, how would the result of this experiment be different?

Har Gobind Khorana and his colleagues performed numerous experiments translating synthetic mRNAs. In one experiment, an mRNA molecule with a repeating UG dinucleotide sequence was assembled and translated.

How did the polypeptide composition help confirm the triplet nature of the genetic code?

Har Gobind Khorana and his colleagues performed numerous experiments translating synthetic mRNAs. In one experiment, an mRNA molecule with a repeating UG dinucleotide sequence was assembled and translated.

Write the sequence of this mRNA and give its polarity.

An experiment by Khorana and his colleagues translated a synthetic mRNA containing repeats of the trinucelotide UUG.

How does the result of this experiment help confirm the triplet nature of the genetic code?

An experiment by Khorana and his colleagues translated a synthetic mRNA containing repeats of the trinucelotide UUG.

What is the result obtained from each reading frame?

An experiment by Khorana and his colleagues translated a synthetic mRNA containing repeats of the trinucelotide UUG.

How many reading frames are possible in this mRNA?