A portion of a DNA template strand has the base sequence
5′-...ACGCGATGCGTGATGTATAGAGCT...-3′
Identify the sequence and polarity of the mRNA transcribed from this fragmentary template-strand sequence.

Several lines of experimental evidence pointed to a triplet genetic code. Identify three pieces of information that supported the triplet hypothesis of genetic code structure.

Assuming the genetic code is a triplet, what effect would the addition or loss of two nucleotides have on the reading frame? The addition or loss of three, six, or nine nucleotides?

The mRNA formed from the repeating tetranucleotide UUAC incorporates only three amino acids, but the use of UAUC incorporates four amino acids. Why?

A portion of a DNA template strand has the base sequence

5′-...ACGCGATGCGTGATGTATAGAGCT...-3′

Which is the third amino acid added to the polypeptide chain?

In studies using repeating copolymers, AC . . . incorporates threonine and histidine, and CAACAA . . . incorporates glutamine, asparagine, and threonine. What triplet code can definitely be assigned to threonine?

What are the differences between the universal code and that found in the mitochondria of some species? Given that some changes (UGA =stop→Trp) have occurred multiple independent times in evolution, can you think of any selective advantage to the mitochondrial code?

In a coding experiment using repeating copolymers, the following data were obtained:

AGG is known to code for arginine. Taking into account the wobble hypothesis, assign each of the four codons produced in the experiment to its correct amino acid.

In the triplet binding technique, radioactivity remains on the filter when the amino acid corresponding to the codon is labeled. Explain the rationale for this technique.