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Semiconservative Replication definitions

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  • DNA Replication
    Cellular process producing identical DNA molecules, ensuring genetic information is passed to daughter cells during division.
  • Semi-Conservative Replication
    Each new DNA double helix contains one parental strand and one newly synthesized strand after replication.
  • Conservative Replication
    Model proposing that the original DNA helix remains intact while an entirely new double helix is formed.
  • Dispersive Replication
    Model suggesting that both DNA helices contain interspersed segments of old and new DNA after replication.
  • Meselson-Stahl Experiment
    Classic study using nitrogen isotopes in E. coli to distinguish between DNA replication models by analyzing DNA density.
  • E. coli
    Bacterial species used as a model organism due to its rapid division and suitability for DNA replication studies.
  • Nitrogen-15
    Heavy isotope incorporated into DNA to label original strands, allowing differentiation from newly synthesized DNA.
  • Nitrogen-14
    Light isotope used to label new DNA strands, enabling visualization of replication patterns in experiments.
  • Density Gradient
    Technique separating DNA molecules based on mass, revealing differences in isotope incorporation after replication.
  • Double Helix
    Structural form of DNA consisting of two intertwined strands, serving as the template during replication.
  • Template Strand
    Original DNA strand used as a guide for synthesizing a complementary new strand during replication.
  • Band
    Visible region in a density gradient tube indicating DNA of a specific mass or isotope composition.
  • Petri Dish
    Shallow laboratory container used to culture bacteria, providing controlled environments for experimental manipulation.
  • Replication Cycle
    Complete sequence of events in which DNA is duplicated, resulting in two DNA molecules from one original.
  • Strand
    Linear sequence of nucleotides forming one half of the DNA double helix, either parental or newly synthesized.