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Ch. 10 - Eukaryotic Chromosome Abnormalities and Molecular Organization
Sanders - Genetic Analysis: An Integrated Approach 3rd Edition
Sanders3rd EditionGenetic Analysis: An Integrated ApproachISBN: 9780135564172Not the one you use?Change textbook
Chapter 10, Problem 10b

A researcher interested in studying a human gene on chromosome 21 and another gene on the X chromosome uses FISH probes to locate each gene. The chromosome 21 probe produces green fluorescent color, and the X chromosome probe produces red fluorescent color.
If the subject studied is male, how many green and red spots will be detected? Explain your answer.

Verified step by step guidance
1
Understand the FISH (Fluorescence In Situ Hybridization) technique: FISH uses fluorescent probes that bind to specific DNA sequences on chromosomes. The green probe binds to the gene on chromosome 21, and the red probe binds to the gene on the X chromosome.
Recall the chromosomal composition of a male: Males have one X chromosome and one Y chromosome (XY), along with two copies of each autosome (non-sex chromosomes, including chromosome 21).
Determine the number of chromosome 21 copies in a male: Since chromosome 21 is an autosome, males have two copies of it. Therefore, the green probe will bind to both copies of chromosome 21, resulting in two green fluorescent spots.
Determine the number of X chromosome copies in a male: Males have only one X chromosome. The red probe will bind to the single X chromosome, resulting in one red fluorescent spot.
Summarize the findings: In a male subject, the FISH analysis will detect two green spots (from chromosome 21) and one red spot (from the X chromosome). This is due to the chromosomal composition of males (XY) and the autosomal nature of chromosome 21.

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Key Concepts

Here are the essential concepts you must grasp in order to answer the question correctly.

Chromosome Structure and Function

Chromosomes are structures within cells that contain DNA, which carries genetic information. Humans have 23 pairs of chromosomes, including one pair of sex chromosomes (XX for females and XY for males). Each chromosome can carry multiple genes, and their arrangement is crucial for proper gene expression and inheritance.
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Chromosome Structure

Fluorescence In Situ Hybridization (FISH)

FISH is a molecular cytogenetic technique used to detect and localize the presence or absence of specific DNA sequences on chromosomes. By using fluorescent probes that bind to specific genes, researchers can visualize the location of these genes under a fluorescence microscope, allowing for the identification of chromosomal abnormalities and gene mapping.
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Functional Genomics

Sex Chromosomes and Gene Dosage

In humans, males have one X and one Y chromosome (XY), while females have two X chromosomes (XX). This difference in sex chromosome composition affects gene dosage; males have one copy of genes on the X chromosome, while females have two. Therefore, in the context of the question, a male subject will show one red spot for the X chromosome gene and two green spots for the gene on chromosome 21, as each chromosome 21 is present in two copies.
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Related Practice
Textbook Question

Mating between a male donkey (2n = 62) and a female horse (2n = 64) produces sterile mules. Recently, however, a very rare event occurred—a female mule gave birth to an offspring by mating with a horse.

How many chromosomes does the mule–horse offspring carry?

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Textbook Question

Mating between a male donkey (2n = 62) and a female horse (2n = 64) produces sterile mules. Recently, however, a very rare event occurred—a female mule gave birth to an offspring by mating with a horse.

Why is it very unlikely that the offspring will have fully horse-like genetic characteristics?

793
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Textbook Question

A researcher interested in studying a human gene on chromosome 21 and another gene on the X chromosome uses FISH probes to locate each gene. The chromosome 21 probe produces green fluorescent color, and the X chromosome probe produces red fluorescent color.

If the subject studied is female, how many green and red spots will be detected? Explain your answer.

489
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Textbook Question

In what way does position effect variegation (PEV) of Drosophila eye color indicate that chromatin state can affect gene transcription?

819
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Textbook Question

A pair of homologous chromosomes in Drosophila has the following content (single letters represent genes):

Chromosome 1RNMDHBGKWU

Chromosome 2RNMDHBDHBGKWU

What term best describes this situation?

545
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Textbook Question

A pair of homologous chromosomes in Drosophila has the following content (single letters represent genes):

Chromosome 1RNMDHBGKWU

Chromosome 2RNMDHBDHBGKWU

Diagram the pairing of these homologous chromosomes in prophase I.

383
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