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Multiple Choice
Which of the following can be used to preserve bacterial cultures?
A
Freezing at -80 ^\(\circ\) C
B
Storing at room temperature in nutrient broth
C
Incubation at 37 ^\(\circ\) C
D
Exposure to ultraviolet light
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Verified step by step guidance
1
Understand the goal: preserving bacterial cultures means maintaining their viability and preventing changes in their characteristics over time.
Evaluate each option based on how it affects bacterial survival and growth: storing at room temperature in nutrient broth and incubation at 37 ^\(\circ\) C both promote bacterial growth but do not effectively preserve cultures long-term.
Consider the effect of ultraviolet light exposure, which damages bacterial DNA and is used to kill or reduce bacteria, so it is not suitable for preservation.
Recognize that freezing at -80 ^\(\circ\) C slows down all metabolic processes and prevents bacterial growth, effectively preserving the cultures for extended periods.
Conclude that freezing at -80 ^\(\circ\) C is the best method among the options for preserving bacterial cultures.