Multiple Choice
In the context of antimicrobial susceptibility testing, when should zones of inhibition be measured?
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1. Introduction to Microbiology
Introduction to Microbiology
Multiple Choice
Which of the following is a commonly used method for antimicrobial susceptibility testing in microbiology laboratories?
A
Gram staining
B
Kirby-Bauer disk diffusion test
C
ELISA (enzyme-linked immunosorbent assay)
D
Polymerase chain reaction (PCR)
Verified step by step guidance1
Understand that antimicrobial susceptibility testing is used to determine the effectiveness of antibiotics against specific bacteria isolated from a patient sample.
Recognize that Gram staining is a staining technique used to classify bacteria based on their cell wall properties, but it does not test antibiotic susceptibility.
Know that ELISA (enzyme-linked immunosorbent assay) is primarily used to detect antigens or antibodies, not to test bacterial susceptibility to antibiotics.
Understand that PCR (polymerase chain reaction) is a molecular technique used to amplify DNA sequences and can detect resistance genes but is not a direct method for susceptibility testing.
Identify that the Kirby-Bauer disk diffusion test is a standardized method where antibiotic-impregnated disks are placed on an agar plate inoculated with bacteria, and zones of inhibition are measured to determine susceptibility.
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