Multiple Choice
Who identified microorganisms as the cause of infection in people?
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1. Introduction to Microbiology
Introduction to Microbiology
Multiple Choice
Which of the following techniques can be used to sterilize microbiological media?
A
Refrigeration at 4 ^\(\circ\) C
B
Incubation at 37 ^\(\circ\) C
C
Filtration with a 10 μm filter
D
Autoclaving
Verified step by step guidance1
Understand the goal: sterilization means the complete elimination of all forms of microbial life, including spores, from the microbiological media.
Evaluate refrigeration at 4 ^\(\circ\) C: this technique slows down microbial growth but does not kill microbes, so it does not achieve sterilization.
Evaluate incubation at 37 ^\(\circ\) C: this temperature is optimal for many microbes to grow, so it cannot sterilize media; instead, it may promote microbial growth.
Evaluate filtration with a 10 \(\mu\) m filter: typical bacteria range from about 0.5 to 5 \(\mu\) m in size, so a 10 \(\mu\) m filter is too large to remove bacteria effectively and thus cannot sterilize the media.
Understand autoclaving: it uses high-pressure saturated steam at around 121 ^\(\circ\) C for a specific time to kill all microorganisms and spores, making it an effective sterilization method for microbiological media.
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