Multiple Choice
Which statement best explains how the loop dilution/pour plate technique results in the isolation of individual colonies?
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10. Dynamics of Microbial Growth
Growing a Pure Culture
Multiple Choice
In microbiology, what best defines a pure culture?
A
A population of microorganisms consisting of a single species or strain, free of contaminating organisms
B
A sample taken directly from a natural environment without laboratory isolation
C
A culture grown only on selective media to inhibit all microbial growth
D
A mixed community of multiple microbial species growing together in the same medium
Verified step by step guidance1
Understand that a pure culture in microbiology refers to a population of microorganisms that are genetically identical, meaning they belong to a single species or strain.
Recognize that a pure culture must be free from any contaminating organisms, ensuring that only one type of microorganism is present.
Differentiate a pure culture from a sample taken directly from a natural environment, which usually contains multiple species and is not isolated.
Note that selective media are used to inhibit the growth of certain microbes but do not guarantee a pure culture by themselves; they help in isolating specific organisms.
Identify that a mixed community contains multiple microbial species growing together, which is the opposite of a pure culture.
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