Multiple Choice
Which of the following immunoassay techniques would be the best for screening thousands of specimens for COVID-19?
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Table of contents
- 1. Introduction to Microbiology3h 27m
- Introduction to Microbiology18m
- Introduction to Taxonomy26m
- Scientific Naming of Organisms9m
- Members of the Bacterial World10m
- Introduction to Bacteria9m
- Introduction to Archaea10m
- Introduction to Eukarya20m
- Acellular Infectious Agents: Viruses, Viroids & Prions19m
- Importance of Microorganisms25m
- Scientific Method27m
- Experimental Design30m
- 2. Disproving Spontaneous Generation1h 18m
- 3. Chemical Principles of Microbiology3h 36m
- 4. Water1h 28m
- 5. Molecules of Microbiology2h 28m
- 6. Cell Membrane & Transport3h 28m
- Cell Envelope & Biological Membranes12m
- Bacterial & Eukaryotic Cell Membranes8m
- Archaeal Cell Membranes18m
- Types of Membrane Proteins8m
- Concentration Gradients and Diffusion9m
- Introduction to Membrane Transport14m
- Passive vs. Active Transport13m
- Osmosis33m
- Simple and Facilitated Diffusion17m
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- ABC Transporters11m
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- Types of Small Molecule Transport Review9m
- Endocytosis and Exocytosis15m
- 7. Prokaryotic Cell Structures & Functions5h 52m
- Prokaryotic & Eukaryotic Cells26m
- Binary Fission11m
- Generation Times16m
- Bacterial Cell Morphology & Arrangements35m
- Overview of Prokaryotic Cell Structure10m
- Introduction to Bacterial Cell Walls26m
- Gram-Positive Cell Walls11m
- Gram-Negative Cell Walls20m
- Gram-Positive vs. Gram-Negative Cell Walls11m
- The Glycocalyx: Capsules & Slime Layers12m
- Introduction to Biofilms6m
- Pili18m
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- Introduction to Prokaryotic Flagella12m
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- Review of Chemicals Used to Control Microbial Growth11m
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- Introduction to Regulation of Gene Expression13m
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- Review of the Lac Operon & Trp Operon11m
- Introduction to Eukaryotic Gene Regulation9m
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- 16. Microbial Genetics4h 44m
- Introduction to Microbial Genetics11m
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- 17. Biotechnology3h 0m
- 18. Viruses, Viroids, & Prions4h 56m
- Introduction to Viruses20m
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- Bacteriophage: Lytic Phage Infections12m
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- 19. Innate Immunity7h 15m
- Introduction to Immunity8m
- Introduction to Innate Immunity17m
- Introduction to First-Line Defenses5m
- Physical Barriers in First-Line Defenses: Skin13m
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- Introduction to Cells of the Immune System15m
- Cells of the Immune System: Granulocytes29m
- Cells of the Immune System: Agranulocytes25m
- Introduction to Cell Communication5m
- Cell Communication: Surface Receptors & Adhesion Molecules16m
- Cell Communication: Cytokines27m
- Pattern Recognition Receptors (PRRs)45m
- Introduction to the Complement System24m
- Activation Pathways of the Complement System23m
- Effects of the Complement System23m
- Review of the Complement System12m
- Phagoctytosis21m
- Introduction to Inflammation18m
- Steps of the Inflammatory Response26m
- Fever8m
- Interferon Response25m
- 20. Adaptive Immunity7h 14m
- Introduction to Adaptive Immunity32m
- Antigens12m
- Introduction to T Lymphocytes38m
- Major Histocompatibility Complex Molecules20m
- Activation of T Lymphocytes21m
- Functions of T Lymphocytes25m
- Review of Cytotoxic vs Helper T Cells13m
- Introduction to B Lymphocytes27m
- Antibodies14m
- Classes of Antibodies35m
- Outcomes of Antibody Binding to Antigen15m
- T Dependent & T Independent Antigens21m
- Clonal Selection20m
- Antibody Class Switching17m
- Affinity Maturation14m
- Primary and Secondary Response of Adaptive Immunity21m
- Immune Tolerance28m
- Regulatory T Cells10m
- Natural Killer Cells16m
- Review of Adaptive Immunity25m
- 21. Principles of Disease6h 57m
- Symbiotic Relationships12m
- The Human Microbiome46m
- Characteristics of Infectious Disease47m
- Stages of Infectious Disease Progression26m
- Koch's Postulates26m
- Molecular Koch's Postulates11m
- Bacterial Pathogenesis36m
- Introduction to Pathogenic Toxins6m
- Exotoxins Cause Damage to the Host40m
- Endotoxin Causes Damage to the Host13m
- Exotoxins vs. Endotoxin Review13m
- Immune Response Damage to the Host15m
- Introduction to Avoiding Host Defense Mechanisms8m
- 1) Hide Within Host Cells5m
- 2) Avoiding Phagocytosis31m
- 3) Surviving Inside Phagocytic Cells10m
- 4) Avoiding Complement System9m
- 5) Avoiding Antibodies25m
- Viruses Evade the Immune Response27m
- 25. Epidemiology2h 24m
- Introduction to Epidemiology37m
- Introduction to Chain of Infection5m
- Reservoirs of Infection12m
- Disease Transmission4m
- Horizontal Disease Transmission30m
- Colonization of Susceptible Host7m
- Factors Influencing Epidemiology11m
- Emerging Infectious Diseases12m
- Healthcare-Associated Infections13m
- Epidemiological Studies8m
- 26. Applications of the Immune Response2h 9m
- 27. Immunological Disorders3h 29m
- 28. Antimicrobial Drugs3h 38m
- Introduction to Antimicrobial Drugs8m
- How Antimicrobial Drugs Work10m
- Broad vs Narrow Spectrum Drugs9m
- Superinfections11m
- Drug Interactions: Synergism and Antagonism8m
- Therapeutic Window & Therapeutic Index6m
- Inhibitors of Cell Wall Synthesis: Beta-lactam & Penicillin36m
- Inhibitors of Cell Wall Synthesis: Polypeptide Antibiotics & Isoniazid8m
- Inhibitors of Protein Synthesis11m
- Disruptors of Cell Membranes11m
- Inhibitors of Nucleic Acid Synthesis9m
- Competitive Inhibitors of Metabolic Pathways12m
- Antifungal Drugs11m
- Antiviral Drugs10m
- Tests to Guide Antimicrobial Use21m
- Antimicrobial Resistance29m
- 29. Microbial Infections - Skin and Eyes46m
- 30. Microbial Infections - Respiratory System1h 23m
- 31. Microbial Infections - Digestive System1h 10m
- 33. Microbial Infections - Nervous System2h 15m
- 34. Microbial Infections - Urogenital System1h 40m
26. Applications of the Immune Response
Immunoassay: Western Blotting
Multiple Choice
All of the following immunoassay testing techniques are paired with their correct characteristics except which of these answers?
A
Fluorescent antibody test – microscopy.
B
ELISA – radioactive label.
C
Western blot – gel electrophoresis.
D
All of the above are matched correctly.
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Verified step by step guidance1
Step 1: Understand the principle behind each immunoassay technique mentioned. The Fluorescent Antibody Test uses fluorescent dyes to label antibodies, which are then visualized under a fluorescence microscope, so it is correctly paired with microscopy.
Step 2: Review the ELISA (Enzyme-Linked Immunosorbent Assay) technique. ELISA uses an enzyme-linked antibody or antigen to produce a color change, not a radioactive label. Radioactive labels are characteristic of radioimmunoassays (RIA), so this pairing is incorrect.
Step 3: Examine the Western blot technique. Western blot involves separating proteins by gel electrophoresis, transferring them to a membrane, and detecting specific proteins with labeled antibodies, so it is correctly paired with gel electrophoresis.
Step 4: Compare all options to identify which pairing is incorrect. Since ELISA does not use radioactive labels, the statement 'ELISA – radioactive label' is the incorrect match.
Step 5: Conclude that the correct answer to the question is the ELISA paired with radioactive label, as it is the only mismatched pair among the options.
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