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Microbiology Metabolism and Photosynthesis Study Guide

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  • Anabolism

    refers to enzyme-regulated energy-requiring reactions that build complex molecules from simpler ones.

  • Catabolism

    is the breakdown of complex molecules into simpler ones, releasing energy.

  • Activation energy

    is the energy required to start a chemical reaction.

  • Competitive inhibitor

    binds to the active site of an enzyme, blocking substrate binding.

  • Noncompetitive inhibitor

    binds to an allosteric site, changing enzyme shape and reducing activity.

  • Photoautotroph

    use light energy to fix carbon dioxide into organic compounds.

  • Chemoautotroph

    obtain energy by oxidizing inorganic molecules and fix CO2 for growth.

  • Chemoheterotroph

    obtain energy and carbon from organic compounds.

  • Photoheterotroph

    use light for energy but require organic compounds as carbon sources.

  • Apoenzyme

    is the protein portion of an enzyme, inactive without its cofactor.

  • Cofactor

    is a non-protein component (metal ion or organic molecule) required for enzyme activity.

  • Coenzyme

    is an organic cofactor that assists enzyme function, often derived from vitamins.

  • Holoenzyme

    is the active enzyme consisting of apoenzyme plus its cofactor(s).

  • Effect of heat and pH on enzymes

    can denature enzymes, altering their shape and reducing or stopping their activity.

  • Feedback inhibition

    occurs when the end product of a pathway inhibits an earlier enzyme to regulate the pathway.

  • Are sulfa drugs competitive or noncompetitive inhibitors?

    Sulfa drugs act as competitive inhibitors by mimicking substrates and blocking enzyme active sites.

  • Three general ways ATP can be produced

    ATP is produced by substrate-level phosphorylation, oxidative phosphorylation, and photophosphorylation.

  • Products of glycolysis

    Glycolysis produces 2 pyruvate, 2 ATP, and 2 NADH. Oxygen is not consumed.

  • Advantages of the Pentose Phosphate Pathway

    It generates 5-carbon sugars for nucleotide synthesis and provides reducing power as NADPH.

  • Products of the Krebs cycle

    The Krebs cycle produces CO2, NADH, FADH2, and ATP. It turns twice per glucose and occurs in the mitochondrial matrix in eukaryotes and cytoplasm in prokaryotes.

  • How NADH and FADH2 energy is converted to ATP

    Energy in NADH and FADH2 is converted to ATP via the electron transport chain and oxidative phosphorylation.

  • Stages producing/consuming CO2, O2, H2O

    CO2 is produced in the Krebs cycle; O2 is consumed in the electron transport chain; H2O is produced at the end of the electron transport chain.

  • Electron transport chain without O2

    The electron transport chain cannot function without O2 as the final electron acceptor in aerobic respiration.

  • Crucial event during fermentation

    Fermentation regenerates NAD+ from NADH, allowing glycolysis to continue without oxygen.

  • By-products of fermentation

    Common fermentation by-products include ethanol and lactic acid.

  • Testing for fermentation products

    Fermentation can be tested by detecting acid or ethanol production using pH indicators or specific chemical tests.

  • How fats enter glycolysis and Krebs cycle

    Fats are broken into glycerol and fatty acids; glycerol enters glycolysis as dihydroxyacetone phosphate, fatty acids undergo beta-oxidation to acetyl-CoA for Krebs cycle.

  • Protein catabolism process

    Proteins are first deaminated, then their carbon skeletons enter glycolysis or Krebs cycle for catabolism.

  • Carbon fixation

    Carbon fixation is the process of converting CO2 into organic molecules during photosynthesis.

  • Products of cyclic photophosphorylation

    Cyclic photophosphorylation produces ATP only; electrons return to chlorophyll.

  • Products of non-cyclic photophosphorylation

    Non-cyclic photophosphorylation produces ATP, NADPH, and releases O2.

  • Why cyclic and non-cyclic photophosphorylation are needed

    Both systems balance ATP and NADPH production to meet cellular energy and reducing power needs.

  • Calvin-Benson cycle

    The Calvin-Benson cycle uses ATP and NADPH to fix CO2 into a 3-carbon sugar (G3P) for carbohydrate synthesis.

  • Intermediates for biosynthesis

    Intermediates from catabolic pathways are used to synthesize triacylglycerides, amino acids, polysaccharides, and nucleosides.