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Multiple Choice
How many net ATP and NADH molecules are produced from one molecule of glucose during glycolysis?
A
2 ATP and 2 NADH
B
2 ATP and 4 NADH
C
4 ATP and 4 NADH
D
4 ATP and 2 NADH
Verified step by step guidance
1
Understand that glycolysis is the process of breaking down one molecule of glucose (C₆H₁₂O₆) into two molecules of pyruvate (C₃H₄O₃) in the cytoplasm of the cell.
Recognize that glycolysis consists of 10 steps, which can be divided into two phases: the energy investment phase and the energy payoff phase.
In the energy investment phase, 2 ATP molecules are used to phosphorylate glucose and its intermediates, preparing them for subsequent breakdown.
In the energy payoff phase, 4 ATP molecules are produced through substrate-level phosphorylation, and 2 NAD⁺ molecules are reduced to 2 NADH molecules as electrons are transferred from intermediates.
Calculate the net gain of ATP by subtracting the 2 ATP used in the investment phase from the 4 ATP produced in the payoff phase, resulting in a net gain of 2 ATP. Additionally, note the production of 2 NADH molecules during glycolysis.