Multiple Choice
In the context of cell communication, which stain is used to stain the background in the capsule stain?
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2. Cell Chemistry & Cell Components
Introduction to Chemical Bonding
Multiple Choice
In the capsule staining procedure, which of the following components is stained first?
A
The bacterial cell
B
The background
C
The capsule
D
The cell wall
Verified step by step guidance1
Understand the purpose of capsule staining: Capsule staining is a differential staining technique used to visualize the capsule of certain bacteria. The capsule is a gelatinous outer layer that can protect bacteria from phagocytosis.
Identify the components involved in the staining process: The components typically include the bacterial cell, the capsule, and the background.
Recognize the order of staining: In capsule staining, the primary stain is applied first to color the bacterial cell. This is often done using a basic dye that adheres to the cell surface.
Consider the role of the background stain: After staining the bacterial cell, an acidic stain is used to color the background. This creates a contrast between the stained cell and the unstained capsule.
Conclude the process: The capsule itself remains unstained and appears as a clear halo around the stained bacterial cell against the colored background. This allows for the visualization of the capsule.
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